As the civil engineering technology development, the structural form selection is more and more important in the design of high-rise building. However, due to the structural from selection of high-rise building is a very complex issue, this paper proposed a kind of method of MATLAB neural network for the structural form selection of the high-rise building and compiled the expert system of the structural form selection of the high-rise building with artificial neural network by MATLAB.The results showed that the method was feasible and could help designers select the appropriate structure.