This paper takes Ningbo Olympic sports center swimming pool project as the research background for large span floor vibration comfort problems of the project, has carried on the analysis and design of long-span floor comfort. Floor of the finite element model is established, considering the working condition of walking, running and jumping, etc. Using time history analysis method for structure under various operating conditions on the acceleration response. Use of ATC is given at the same time would be acceptable to different environmental human comfort horizontal peak ground acceleration as the comfort evaluation standard. The calculation results show that the objective of the large-span floor with appropriate stiffness and comfort. This paper uses the calculation method and evaluation standard of comfort analysis of the same kind of large span floor has a certain reference value.